Dear Sir,
Yes, you can walk at 1 am which will not be a problem. It is absolutely safe. Luggage can be deposited well before starting http://gotirupati.com/tirumala-ttd-free-luggage-counter/

Please visit Srinivasam complex to take bath.

Here is the procedure to be followed for Tirumala

1) Before taking steps path, plan to visit Kapila Theertham in Tirupati, wash your feet and hand and sprinkle water on you head from this pushkarini http://gotirupati.com/kapila-theertham-temple-history/
2) Then you can start journey to Tirumala, after reaching Tirumala, offer hair at Rambagicha Guest House near main temple. After taking bath at Rambagicha Guest House.Then plan to visit Swamy Pushkarini (Srivari Pushkarini) near to main Temple, wash your hands and feet and sprinkle water on your head from this pushkarini. The visit Aadhi Varaha swamy Temple which is at the bank of Pushkarini.
http://gotirupati.com/holiness-swamy-pushkarini/
http://gotirupati.com/aadhi-varahaswamy-temple/

3) After that you are good to join Darshan line for Lord Venkateswara Swamy Darshan
4) After completing Darshan, you can plan to visit Sri Padmavathi Amma Varu Temple Tiruchanoor

Please let me know if you need more details
Thanks,